https://www.proz.com/kudoz/english-to-russian/medical-pharmaceuticals/7148629-faecal-stem-solution.html
Aug 21, 2023 15:39
9 mos ago
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English term

faecal stem solution

English to Russian Medical Medical: Pharmaceuticals clinical trials
Male C57BL/6 mice were i.p. injected with a faecal stem solution to induce abdominal peritonitis 30 min prior to receiving either ascorbic acid (200 mg/kg) or dehydroascorbic acid (200 mg/kg).

Proposed translations

34 mins
Selected

суспензия стволовых клеток эпителия кишечника

Не стоит переводить solution как "раствор" - клетки суспендированы, а не растворены, слишком велики для образования истинных растворов.

https://repo.odmu.edu.ua/xmlui/bitstream/handle/123456789/13...
...3) крысы с модельным паркинсонизмом, которым с лечебной целью одноразово внутривенно вводи-ли 0,5 мл суспензии стволовых клеток в дозе 2×10 6
на 1 крысу.

https://studfile.net/preview/9830912/page:17/
...Эти данные свидетельствуют, что восстановление эпителиального слоя слизистой оболочки у выживших животных протекает очень быстро, а пул стволовых клеток кишечного эпителия в этот период находится на стадии гиперрегенерации
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6 days

базовый фекальный раствор

базовый (основной) фекальный раствор

Это раствор, приготовленный из фекальных масс, вводимый (без дальнейшего разведения) внутрибрюшинно для индукции перитонита.

https://pubmed.ncbi.nlm.nih.gov/22523283/
Male C57BL/6 mice (weight 25–30
g, age 8 wk) were obtained from Jackson Laboratory (Bar Harbor,
ME). Polymicrobial sepsis (peritonitis) was induced by intraperitoneal
(i.p.) introduction of fecal stem solution into the peritoneum (FIP).
Fecal stem solution was prepared by a modification of the method
reported by Bernardshaw et al. (8). Dry fecal pellets were randomly
collected from all the mice-containing cages 1 day before the challenge. Weighed pellets (70 pellets 1 g) were pooled and suspended
in saline (180 mg feces/1 ml saline). The solution was capped tightly,
vortexed for 5 min, and stored overnight at 4°C. The mixture was then
allowed to return to room temperature and solids were compacted by
low-speed centrifugation (50 g) for 5 min. The recovered supernatant
was termed fecal stem solution.

Этот базовый (основной) раствор может вводиться в разведениях, как у Bernardshaw et al. (8)
https://pubmed.ncbi.nlm.nih.gov/16670646/
Preparation of Standard Fecal Solution
The inoculum was made by pooled dry fecal pellets that were randomly collected
from all the mice-containing cages 1 day before the challenge. Within 30 min of
challenge, 10 mL of pooled pellets, weighing 4.0 T 0.5 g, were mixed with 30 mL of
PBS at room temperature. The mixture was thoroughly shaken before filtering
through a double layer of surgical gauze with a mesh diameter of 1 to 2 mm. This
stem solution was defined as the undiluted fecal inoculum (1/1)
. Further dilutions
were made by adding PBS.
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