Glossary entry

English term or phrase:

anti-CH1 affinity batch binding

Russian translation:

аффинная батч-очистка на смоле с иммобилизованными анти-CH1

Added to glossary by Nitrogen
Sep 26, 2020 08:02
3 yrs ago
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English term

anti-CH1 affinity batch binding

English to Russian Science Biology (-tech,-chem,micro-) иммунология�
Purification was performed via ***anti-CH1 affinity batch binding*** (CaptureSelect IgG-CH1 Affinity Matrix, Thermo-Fisher Scientific, Waltham, MA, USA) or Protein A (rProteinA Sepharose, Fast flow, GE Healthcare, Uppsala, Sweden) batch binding using 1ml resin/100 mL supernatant.

Proposed translations

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Selected

аффинная батч-очистка на смоле с иммобилизованными анти-CH1

Batch method purification can be performed at any scale. However, it is most commonly reserved for microcentrifuge tube scale purifications involving 10-200 µl of resin. In batch method purification, wash and elution fractions are separated from the resin after centrifuging to pellet the resin beads.
http://tools.thermofisher.com/content/sfs/brochures/TR0004-B...

По сути, батч-очистка - это не колоночная хроматография, а связывание со смолой в пробирке с последующим отделением смолы путем центрифугирования.

Пример:
Со временем произошёл переход от батч-метода выделения белков к хроматографии.
http://blood.ru/index.php?option=com_content&view=article&id...
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