V(D)J reporter assay
Polish translation: test reporterowy V(D)J
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| 09:02 Mar 5, 2022 |
| English to Polish translations [PRO] Science - Biology (-tech,-chem,micro-) | |||||||
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|  Selected response from: Frank Szmulowicz, Ph. D. United States Local time: 21:26 | ||||||
Grading comment
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| 2 | test reporterowy V(D)J |
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| Reporter Gene Assays |
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Answers
3 hrs confidence: 
| v(d)j reporter assay test reporterowy V(D)J Explanation: Chociaż nie opublikowano żadnych dowodów na to, że SARS-CoV-2 może infekować tymocyty lub komórki limfoidalne szpiku kostnego, nasz test reporterowy V(D)J in vitro pokazuje, że białko wypustek intensywnie utrudnia rekombinację V(D)J. https://psnlin.pl/artykuly,bialko-spike-hamuje-naprawe-uszko... cccccccccc przeprowadziliśmy test reporterowy lucyferazy, który potwierdził oddziaływanie miR-487a-. 3p z mRNA CTLA4 i FOXO3 https://igcz.poznan.pl/wp-content/uploads/2015/11/Agnieszka_... ccccc Został opracowany oryginalny test reporterowy umożliwiający badanie znaczenia polimorfizmów i mutacji występujących w intronach. https://studylibpl.com/doc/1065617/streszczenie |
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Reference comments
2 hrs |
| Reference Reference information: assay - analysis, test The Reporter's Test requires the patient to verbally report to a hypothetical third person the actions the examiner is performing on an array of tokens, so as to enable him to replicate them. - https://pubmed.ncbi.nlm.nih.gov/679709/ VDJ stands for variability, diversity, and joining, respectively, and VDJ rearrangement has 4 key characteristics that help ensure that each antigen receptor is unique. - https://www.osmosis.org/learn/VDJ_rearrangement variable (V), joining (J), and in some cases, diversity (D) gene segments. - https://en.wikipedia.org/wiki/V(D)J_recombination variable (V) regions, genetically encoded - the same link Joining (J) gene segments. - idem -------------------------------------------------- Note added at 3 horas (2022-03-05 12:36:02 GMT) -------------------------------------------------- Definition of readout (recombination) - https://www.merriam-webster.com/dictionary/readout |
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3 hrs |
| Reference: Reporter Gene Assays Reference information: Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for measuring activation of cell signaling pathways. In these assays, the reporter gene acts as a surrogate for the coding region of the gene under study. The reporter gene construct contains one or more gene regulatory elements being analyzed, the structural sequence of the reporter gene, and the sequences required for the formation of functional mRNA. Following introduction of the reporter construct into cells and experimental treatment, expression levels of the reporter gene are monitored by quantitating the reporter protein enzymatic activity, which is used to characterize regulatory sequences, transcription factor activity, cell signaling, or signaling pathways. https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/reporter-gene-assays.html#:~:text=Reporter%20ge |
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